bio rad proteontm nlc sensor chip (Bio-Rad)

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Bio-Rad bio rad proteontm nlc sensor chip
Bio Rad Proteontm Nlc Sensor Chip, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+proteontm+nlc+sensor+chip/ProteOn+NLC+Sensor+Chip/10__3390_slash_molecules21121632-218-26-26
Average 93 stars, based on 91 article reviews

bio rad proteontm nlc sensor chip - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Expression, Purification, and Characterization of Interleukin-11 Orthologues
Article Snippet: Surface plasmon resonance (SPR) measurements were performed at 25 ◦C using Bio-Rad ProteOnTM XPR36 instrument (Hercules, CA, USA). .. Biotinylated rIL-11 was used as a ligand: 30 μg/mL rIL-11 in a running buffer (PBS, 0.05% TWEEN 20, pH 7.4) was immobilized on the surface of Bio-Rad ProteOnTM NLC sensor chip covered by NeutrAvidin (up to 2000 resonance units, RUs). sIL-11Rα was used as an analyte: 5–80 nM of the protein in the running buffer flowed over the chip at rate of 30 μL/min for 350 s, followed by flushing the chip with the running buffer for 3600 s. The double-referenced SPR sensograms were fitted according to a heterogeneous ligand model, which assumes existence of two populations of the ligand (‘L1‘ and ‘L2’) that bind single analyte molecule (‘A’): L1 + A Kd1←→ kd1 L1 A; L2 + A Kd2←→ kd2 L2 A (1) where Kd and kd refer to equilibrium and kinetic dissociation constants, respectively. .. Kd, kd and Rmax (maximum response) values were evaluated using Bio-Rad ProteOn ManagerTM v.3.1 software.

Article Title: Expression, Purification, and Characterization of Interleukin-11 Orthologues
Article Snippet: Surface plasmon resonance (SPR) measurements were performed at 25 °C using Bio-Rad ProteOnTM XPR36 instrument (Hercules, CA, USA). .. Biotinylated rIL-11 was used as a ligand: 30 μg/mL rIL-11 in a running buffer (PBS, 0.05% TWEEN 20, pH 7.4) was immobilized on the surface of Bio-Rad ProteOnTM NLC sensor chip covered by NeutrAvidin (up to 2000 resonance units, RUs). sIL-11Rα was used as an analyte: 5–80 nM of the protein in the running buffer flowed over the chip at rate of 30 μL/min for 350 s, followed by flushing the chip with the running buffer for 3600 s. The double-referenced SPR sensograms were fitted according to a heterogeneous ligand model, which assumes existence of two populations of the ligand (‘L 1 ‘ and ‘L 2 ’) that bind single analyte molecule (‘A’): (1) L 1 + A ↔ k d 1 K d 1 L 1 A ; L 2 + A ↔ k d 2 K d 2 L 2 A where K d and k d refer to equilibrium and kinetic dissociation constants, respectively. .. K d , k d and R max (maximum response) values were evaluated using Bio-Rad ProteOn ManagerTM v.3.1 software.

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